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Recent reports have suggested that tumors are organized in heterogeneous cell populations and that the capability to initiate malignancies including leukemia and solid tumors resides in only a small subset of cancer cells called cancer stem cells. In the case of human hepatocellular carcinoma (HCC), there are earlier studies showing HCC is originated and propagated from a small number of undifferentiated tumorigenic cells, which express CD133 as a tumorigenic
marker in brain and prostate cancer. In this paper, we confirmed that the CD133+ cells, from a HCC cell line (Huh-7), isolated by MACS possessed high tumorigenecity and clonogenicity as compared to CD133- HCC cells. The differences between CD133+ cells and CD133- cells purified from Huh-7 were also analyzed via 2-DE proteome analysis. The differentially expressed proteins
were analyzed with ImageMaster 2D Platinum 6.0 software and then identified by ESI-Q-TOF MS/MS. Among the identified proteins, certain proteins related to carcinogenesis and metastasis need be confirmed with Western blotting in a further study.