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영어

Hyperglycemia characterized T2M due to a combination of insulin resistance and damaged insulin secretion. In this study, we investigated the possible protective effects of vitamin A as an antioxidant in preventing the development of hyperglycemia. In addition, to compare differences in the rat liver proteome, we have utilized two-dimensional electrophoresis (2-DE). In the present study, male SD rats (n=21, 5 weeks old) were divided into 3 groups: GroupⅠ, normal group; group Ⅱ, diabetic group (STZ, 45 ㎎/㎏ HFD/STZ); group Ⅲ, diabetic group treated with Vitamin A (addition of 83.3 IU/Kg/day). We measured glucose/insulin concentration, body weight and cholesterol to confirm induction of T2M and measured GOT, GPT and liver-to-body weight ratio to confirm liver injury. As a result, vitamin A can be effective at protecting from the liver injury that occurs in T2M. In addition, proteins which were differentially expressed with statistical significance (p < 0.05) in control & diabetes and diabetes & diabetes treated with vitamin A groups were selected and identified by ESI-Q-TOF MS/MS. These proteins might play a key role in the developtment and recovery of liver injury in T2M.