원문정보
초록
영어
To support and enhance the in vitro growth and activity of mesenchymal stem cells(MSCs), the cell culture medium may be supplemented with various proteins and factors in order to mimic the physiological environment in which cells optimally proliferate and differentiate. The effects of mechanical factors on cellular metabolic responses were investigated experimentally using a bioreactor. The effects of various chemical factors, such as growth factors, cytokines, and hormones, on cellular metabolic responses were also investigated. Based on previous reports demonstrating the important roles of mechanical factors in the growth and activity of MSCs, we sought to evaluate the effects of mechanical stimuli on the proliferation of bone marrow-derived MSCs using a cell training bioreactor that imposed cyclic mechanical stretch with parameters of 240 min/day, 0.03 Hz, 5-15% strain. The application of cyclic stretch (5%-15% strain) to MSCs enhanced their proliferation during the early stage (3 days) but not during the late stage (14 days) of batch culture. Mechanical stretch did not increase the release of lactate dehydrogenase
(LDH) from MSCs during culture. Appropriate levels of mechanical stretch (5-10% strain)
increased collagen synthesis but did not alter MSC surface antigen expression. It is thought that the appropriate level of mechanical stretch served