earticle

영어

Human prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP), with comprehensive homology to glandular kallikrein, are representative serum biomarkers of prostate cancer1). Dendritic cell (DC), which is the potent antigen-presenting cells in the immune system, can induce strong T cell responses against viruses, microbial pathogens, and tumors2). Therefore, the immunization using DC loaded with tumor-associated antigens is a powerful method for inducing anti-tumor immunity. The CTP (Cytoplasmic Transduction
Peptide) technology developed by Creagene which can transport attached bio-polymers like nucleic acids or proteins into the cell with high permeation efficiency3). As the active forms of PSA and PAP can mediate apoptotic processing4), we used multimeric forms of prostate antigens (PAs) as an inactive form for antigen pulsing of DCs. In this study, multimeric forms of hCTP-PAs were obtained according to the advanced purification process and subsequently
confirmed by gel filtration chromatography, western blot and Dynamic Light Scattering. Therefore, CTP-conjugated PA multimers transduce into the cytoplasm were efficiently presented on the cell surface without any harm effect on cells via MHC class I molecules and result in induction of a large number of effector cells.