earticle

영어

Acellular dermal matrix(ADM) is a dermal graft material produced from human cadaver skin by decellularization in special buffered solutions containing detergents and enzymes(1). It retains intact basement membrane complex, collagen bundle architectures and the dermal vasculatures. Since 1992 it has been used for the dermal coverage in burns, facial reconstructions, and the soft tissue augmentation(2). Viral safety is a prerequisite for manufacturing the ADM, because human skin might have carried endogenic pathogen or extrinsic
infection in the processes. Cytomegalovirus is the most common human pathogen found in donated human skin. In order to improve the viral safety of ADM, solvent/detergent(S/D) treatment process using tri-n-bytyl phospahate and deoxycholic acid was developed as the decellularization step as well as viral inactivation step. No substantial changes were observed in the physical and biochemical characteristics of the ADM compared with those of the ADM
decellularized with only the detergent, deoxycholic acid. Bovine herpesvirus(a model virus for cytomegalovirus), bovine viral diarrhoea virus(model virus for human hepatitis C virus), and human immunodeficiency virus were completely inactivated to below detectable levels by the S/D treatment process. These results indicate that S/D treatment improves the viral safety of ADM, without destroying the physical and biochemical characteristics.