earticle

영어

Mesenchymal stem cells (MSC) are of great therapeutic potential because of their ability to self-renew and differentiate into multiple tissues. However, the ratio of hMSCs in adult bone marrow is about one per one-hundred-thousand nucleated cells, and the volume of bone marrow obtainable is limited. In addition, after a long period of normal growth, MSC culture showed typical abnormalities of the Hayflick model of cellular senescence in cultured human fibroblasts. The cells varied in size and shape, the cytoplasm began to be granular with many cell inclusions, and debris was formed in the medium. It is known that when the cells become senescent, they are unable to proliferate further. In vitro culture has proven that environmental conditions and differing treatment significantly increase or decrease culture life span. So we confirmed the changes of hMSC senescence during long-term culture using various tools
for proliferation study such as morphological changes, growth kinetics, β -galactosidase staining, BrdU assay and RT-PCR. And we performed proteome profiling to find senescence associated regulators.