원문정보
초록
영어
The immobilization of lipase from Candida rugosa, using ionic liquids as additives to protect the inactivation of lipase by released alcohol and shrinking of gel during sol-gel process, was investigated. The influence of various factors in the sol-gel process on the activity and stability of immobilized lipase was also studied. The highest hydrolytic activity of immobilized lipase was obtained when the most hydrophilic ionic liquid, [C2mim][BF4], was used as an additive,
while the highest stability of immobilized lipase was obtained by using the most hydrophobic ionic liquid, [C16mim][Tf2N]. Therefore, the binary mixtures of these ionic liquids as additives were used to increase both activity and stability of immobilized lipase. The hydrolytic and esterification activities of lipase coimmobilized with the mixture of 1 : 1 at molar ratio were 10-fold and 14-fold greater than in silica gel without ILs, respectively. After 5 days incubation of
this immobilized lipase in n-hexane at 50 , the residual ℃ activity was 84% of initial activity, while the residual activity of the lipase immobilized without ILs was 28%.