원문정보
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영어
Skin whitening effect of Angelica gigas extract has been analyzed. Angelica gigas extract was obtained at pressure of 300 bar and temperature of 60℃ by supercritical carbon dioxide extraction system. To decide the proper treatment concentration of extract, MTT assay was performed with B16-F1 Melanoma.
Angelica gigas extract was showed cytotoxicity at over 10 mg/L concentration. Tyrosinase IC50 value of Angelica gigas extract was 49.89 mg/L, the same as arbutin (49.52 mg/L) which is well known skin whitening material.
Melanogenesis inhibitory effect with melanoma was showed high activity at concentrations of over 0.5 mg/L. Regulation of proteins related to melanogenesis by Angelica gigas extract was investigated using proteome analysis. The proteome of malanoma with or without Angelica gigas extract were profiling by 2D-PAGE. In conclusion, it was shown that many protein factors related to melanogenesis were down-regulated by Angelica gigas extract1,2).