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초록
영어
Metabolic substrate-based cell surface engineering techniques, where exogenously supplied N-acetylmannosamine (ManNAc) analogues are utilized by the sialic acid biosynthetic pathway, allow the cell surface to be endowed with novel physical and chemical properties. Among the ManNAc analogues, it has been reported that Ac5ManNTGc enables thiols to be expressed in cell surface non-natural sialic acids.1) Herein we modified the cells expressing this versatile
functional group (-SH) and immobilized them to a solid substrate. Immobilization has been recognized as an essential element technology of cell-based microchip device manufacturing. The Ac5ManNTGc, which expressed the thiols on cell surface, was incubated with Jurkat (human T lymphoma) cells and we observed that cells were clustered with themselves above time. Cell surface thiols were evaluated by labeling with maleimide-biotin followed by avidin-conjugated quantum dot staining. We prepared the electrochemically active polymer film
containing hydroquinone moiety on ITO (indium tin oxide) electrodes. Cells were immobilized to the polymer film via electrochemical oxidation of hydroquinone moiety. These results can be expected to contribute the site-selective nano/micro immobilization technology of biomolecules including cells.