원문정보
초록
영어
Magnetic particles (MPs) carrying an immobilized affinity ligand have been successfully used in the purification and separation of several biomolecules from biological mixtures such as crude cell lysates, whole blood, etc [1]. In these techniques, there is no need for a prior step such as centrifugation and filtration for solids removal from the suspensions [2]. So the advantages include separation efficiency, simplicity, low cost, and easy scale-up ability and automation. Hepatitis B surface antigen (HBsAg) and anti-HBsAg antibody were the model ligand and capturing molecules for this study. Anti-HBsAg antibody is used for treatment of Hepatitis B virus (HBV) and detection of HBsAg in the serum as a immunosensor [3]. 255 umol of HBsAg was immobilized by covalently linking onto amine-coated 1 mg of MPs. Molar ratio of immobilized
HBsAg to bound anti-HBsAg antibody was 0.58, and these HBsAg-immobilized MPs can be used to purify anti-HBsAg antibody from mixtures including impurities. This method is expected to be highly cost-effective as a single-step purification of various antibodies or proteins.