원문정보
초록
영어
Valiolamine is an important chemical intermediate for the synthesis of voglibose, antidiabetic medicine and has been also isolated from the fermentation broth of Streptomyces hygroscopicus subsp.. Nowadays, intensive effort has been made to produce valiolamine by the fermentation of Streptomyces cells using metabolic engineering tool. Quantitative analysis of valiolamine is crucial for quality control of voglibose production process or monitoring the fermentation process to produce valiolamine. Valiolamine has no significant UV absorption and thus derivatization by chromophoric reagents can increase the detection sensitivity.
This study was aimed to develop a rapid, accurate and precise HPLC method for UV detection of valiolamine following pre-column derivatization. The performance of several derivatization methods were evaluated for valiolamine analysis. The electrospray ionization-mass (ESI-MS) analysis was performed to identify the new peak after derivatization. The experimental conditions for derivatization and separation of valiolamine derivatives were optimized to increase sensitivity.