earticle

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Autonomous bottom-up fabrication based on molecular recognition1 is a conceptually attractive and potentially powerful approach to engineering structures and devices at the molecular scale. DNA is a versatile construction material that can be programmed to self-assemble into nanoscale structures based on specific complementarity between bases.2-4 Here, our research effort aims at developing bio-inspired nanomaterials capable of detecting single nucleotide polymorphism (SNP). About 0.1% single nucleotide variation is observed in human population, leading to challenges in achieving effective medical treatment of different individuals. Considering the importance of SNP in individual drug efficacy, hugh research efforts have been focused on
coming up with fast and convenient SNP detection methods. We developed a novel SNP biosensor material made of DNA-programmed protein nanostructures of which disassembly of the whole structure is triggered by a target DNA oligonucleotide carrying a specific sequence. We designed DNA sequences incorporated in the sensor materials in a way that even a single
base mutation in the target DNA (e.g. a single mutation in a 24-mer DNA oligonucleotide) effectively prevents the protein nanostructures from being disassembled. We could detect SNP by monitoring structural changes in the protein nanostructure. The SNP biosensor material is promising for fast identification of SNP in genes of interest, which enables patient-tailored drug
theraphy.