원문정보
초록
영어
BSA is normally used as a blocking agent in microarrays. However, it will cause the immobilized peptides on a chip to become slightly interactive with macromolecules such as enzymes and antibodies. We developed a recombinant protein-fused substrate (PFS) which can be constantly produced at a reasonable quantity with a small outlay.
Using the mep45 gene encoding the 45 kDa major envelope protein (Mep45) of Selenomonas ruminantium, we cloned a protein-fused substrate (AAKIQASFRGHMARKK-Mep45) for protein kinase C (PKC). In the present study, we describe a suitable strategy for the detection of phosphorylation of a PFS catalysed by PKC by using a powerful radioisotope detection technique in protein microarrays. Further, it can be a useful tool for a high throughput screening and for
biological and medical research.