원문정보
초록
영어
An electrochemical method for glycated hemoglobin (HbA1c) analysis was developed. Gold electrodes was functionalized with boronic acid to prepare HbA1c sensitive surfaces. Boronic acid is known to form covalent bonds between its diol group and cis-diol group of the carbohydrate chain from glycoproteins. In order to modify boronic acid on electrode surface, a conjugation reaction of 3-formylphenyl boronic acid and cystamine were performed (Cys-FPBA). Glycated hemoglobin and glucose oxidase were immobilized onto the boronic acid modified electrode through its carbohydrate moiety under the competition reaction format. With increasing concentration of HbA1c from 2.5% to 15 % of total hemoglobin, a linear signal increase was registered. The limit of detection (LOD) is around 4%. Using this method, HbA1c could be analysed without pre-treatments and detection labels. It is not only expected to analyze HbA1c according to its concentration, but also a variety of glycoproteins that contain peripheral sugar
groups could be applied.