원문정보
초록
영어
In this work, we first report self-assembly of insulin monomers into amyloid fibrils on a solid surface within microchannel by using microfluidics. To demonstrate amyloid formation and growth on a solid surface, we prepared NHS-activated glass and PDMS film embossed with a microchannel. According to the analysis with optical and fluorescence microscope, amyloid aggregates within microchannels were found to be increased with the duration of incubation
due to the continuous flow of insulin monomers. We confirmed numerous amyloid fibrils grown inside microchannels through AFM analysis and the density of fibrils was much higher than those formed in other bulk systems. We observed the formation of spherulites showing “maltese cross” patterns inside the microchannel after 12 h. These results indicate that the microfluidic
self-assembly into amyloid fibrils enables the low consumption of reagents, reduction in incubation time for amyloid formation, and a simultaneous detection.
The microfluidic system for amyloid formation on the surface has a potential contribution in the field of study of the amyloidogenic peptides that are responsible for different neurodegenerative disease.