원문정보
초록
영어
The surface plasmon resonance (SPR) imaging technique has been used to characterize thin organic and biopolymer films at metal interfaces in a spatially resolved manner. Because this approach enables the measurement of interactions between unlabeled biological molecules and surface-bound species in an array format, SPR imaging is thought to be well-suited not only for DNA and protein microarrays but also for small-molecule microarrays. The biosensor for detection of small molecules or diagnosis markers had been required high sensitivity. One of the
methods for enhancing sensitivity was surface modification to provide bio-adaptable space. In this study, we modified the surface of bare gold chip by using our NHS-SAM for enhancing of detection sensitivity. We immobilized the antibody on NHS surface by covalent bonding. And the target antigen were immobilized on modified surface using microarrayer. We observed images of these spots and compared with image of immobilized proteins on 2D surface. These results
demonstrate that SPR imaging is well suited to study biomolecular interactions.