원문정보
초록
영어
The ability to organize multiple biomolecules on micro/nanometer scale pattern is crucial for the development of biological integrated devices such as DNA/protein chip. In this study, a gold pattern on silver substrate was prepared. A novel and ultrasensitive detection method of prostate specific antigen (PSA) by scanning tunneling microscopy (STM) was developed on the micro gold pattern. PSA is a marker for prostate cancer and it has been identified as a potential marker for breast cancer in women. The detection method is based on the change of tunneling current given by the gold nanoparticle dispersed on micro gold pattern insulated with biomaterial. Biomolecular thin film on the pattern and the Au nanoparticle-antibody conjugate were fabricated in an oriented manner with the antibody fragments. As a result, the frequency of current peaks was generated in accordance with the surface density of the dispersed Au nanoparticle on pattern. And, the change of the power spectrum was observed in accordance with the concentration of PSA molecule. The lowest detection limit of the assay system for PSA was 33fM (10pg/mL).