원문정보
초록
영어
Recently, many commercial electronic noses have been developed and are being applied to such areas as food, beverage, agriculture, health, industry and environment. But the electronic noses have limitation; less sensitivity, high cost, large equipment and so on. Biosensor using olfactory receptor can overcome these limitation having high sensitivity and specificity. In previous study, quartz crystal microbalance (QCM) was used to check olfactory receptor protein ODR10 interact with diacetyl. Such system could be applied to test the interaction of human olfactory receptor and specific odorants. For this experiment, Human olfactory receptor OR52D1 interacting with ethyl isobutyrate is chosen. OR52D1 was expressed in E.coli as a fusion protein with GST tag and the expression was confirmed through western blot using anti-GST antibody. Crude membrane extract of the expressed protein was coated on the surface of gold, and the interaction of the olfactory receptor and specific odorant was examined. OR52D1 interacted with isobutyrate stronger than other odorants is confirmed by QCM.