원문정보
초록
영어
Cell biosensor can be used to acquire functional information of the effect of a stimulus on a living system and detect various chemical compounds. An efficient strategy was developed here for immobilizing cells onto the gold surface by cell surface-expression of gold binding polypeptide (GBP). Additionally, core streptavidin (cSA) of Streptomyces avidinii was used as a model protein to demonstrate the effective immobilization of GBP-fusion proteins onto the gold surface. All specific binding of cells and other compounds to the gold surface were detected by surface
plasmon resonance (SPR). As a series of data revealed, cells displaying GBP as well as GBP-fusion proteins bound to the gold surface stably and also, successful interaction between GBP-cSA pre-immobilized onto the gold surface and biotin-horse radish peroxidase (HRP) was observed. Mediated by GBP-fusion protein, whole cell biosensor system for biomolecular interaction studies could be obtained by effective immobilization of bioactive cells onto the gold surface without surface modification. [This work was supported by the KOSEF through the Center for Ultramicrochemical Process Systems, and by grant No. (R01-2006-000-11175-0) from the Basic Research Program of the Korea Science and Engineering Foundation.]