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Enhanced cellular delivery and transfection efficiency of plasmid DNA using positively charged biocompatible colloidal gold nanoparticles

원문정보

Sang Myoung NOH, Won-Ki KIM1, Sun Jae KIM2, Jung Mogg KIM3, Kwang-Hyun BAEK, Yu-Kyoung OH4

한국생물공학회 한국생물공학회 학술대회 2007 춘계학술대회 및 국제심포지움 2007.04 pp.5-5
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초록

영어

Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this study, we report an enhancement of the transfection efficiency of plasmid DNA, via the use of positively charged colloidal gold nanoparticles (PGN). Plasmid DNA encoding for murine interleukin-2 was complexed with PGN at a variety of ratios. The delivery of pVAXmIL-2 into C2C12 cells was dependent on the complexation ratios between PGN and plasmid DNA, presented the highest delivery at a ratio of 2400:1. After complexation with DNA,
PGN showed significantly higher cellular delivery and transfection efficiency than did the polyethylenimines (PEI) of different molecular weights. PGN resulted in a cellular delivery of pVAXmIL-2 6.3-fold higher than PEI25K. The PGN/DNA complex resulted in 3.2- and 2.1-fold higher murine IL-2 protein expression than PEI25K/DNA and PEI2K/DNA complexes. Following i.m. administration, PGN/DNA complex showed more than 4 orders of magnitude higher expression levels as compared to naked DNA. The results of this study suggest that the PGN/DNA complexes may harbor the potential for efficient and safe gene delivery vehicles

키워드

저자정보

  • Sang Myoung NOH Graduate School of Life Science and Biotechnology, Pochon CHA University, Seoul, Korea. 1Division of NanoSciences, Ewha Woman University, Seoul, Korea. 2Department of Nanoengineering, Sejong University, Seoul, Korea. 3College of Medicine, Hanyang University, Seoul, Korea. 4School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
  • Won-Ki KIM1 Graduate School of Life Science and Biotechnology, Pochon CHA University, Seoul, Korea. 1Division of NanoSciences, Ewha Woman University, Seoul, Korea. 2Department of Nanoengineering, Sejong University, Seoul, Korea. 3College of Medicine, Hanyang University, Seoul, Korea. 4School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
  • Sun Jae KIM2 Graduate School of Life Science and Biotechnology, Pochon CHA University, Seoul, Korea. 1Division of NanoSciences, Ewha Woman University, Seoul, Korea. 2Department of Nanoengineering, Sejong University, Seoul, Korea. 3College of Medicine, Hanyang University, Seoul, Korea. 4School of Life Sciences and Biotechnology, Korea University, Seoul, Korea
  • Jung Mogg KIM3 Graduate School of Life Science and Biotechnology, Pochon CHA University, Seoul, Korea. 1Division of NanoSciences, Ewha Woman University, Seoul, Korea. 2Department of Nanoengineering, Sejong University, Seoul, Korea. 3College of Medicine, Hanyang University, Seoul, Korea. 4School of Life Sciences and Biotechnology, Korea University, Seoul, Korea
  • Kwang-Hyun BAEK Graduate School of Life Science and Biotechnology, Pochon CHA University, Seoul, Korea. 1Division of NanoSciences, Ewha Woman University, Seoul, Korea. 2Department of Nanoengineering, Sejong University, Seoul, Korea. 3College of Medicine, Hanyang University, Seoul, Korea. 4School of Life Sciences and Biotechnology, Korea University, Seoul, Korea
  • Yu-Kyoung OH4 Graduate School of Life Science and Biotechnology, Pochon CHA University, Seoul, Korea. 1Division of NanoSciences, Ewha Woman University, Seoul, Korea. 2Department of Nanoengineering, Sejong University, Seoul, Korea. 3College of Medicine, Hanyang University, Seoul, Korea. 4School of Life Sciences and Biotechnology, Korea University, Seoul, Korea

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