원문정보
초록
영어
It is very critical to develop the surface plasmon resonance (SPR) sensor in order to achieve a severe acute respiratory syndrome (SARS) coronavirus sensor. This work is specifically focused on the development of biosensor system to obtain recombinant proteins with the gold-specific affinity. The strategy is based on the principle that the gold binding polypeptide (GBP) as a fusion partner specifically binds onto the gold surface for immobilizing proteins. The SARS coronavirus envelope was employed as a model protein for the specific immobilization of GBP-fusion protein. The fusion proteins were successfully immobilized on the gold surface and examined by SPR analysis. We also analyzed the immobilization efficiency of intracellular protein on the micro-patterned gold surface. Finally, the GBP-fusion method immobilized proteins onto the gold surface without surface modification and in a bioactive form, which is suitable for studying interactions of protein-protein, and other biomolecules [This work was supported in part by MIC & IITA through IT Leading R&D Support Project.].