원문정보
초록
영어
Olfactory receptors (ORs) are the largest member of the G-protein-coupled receptors which mediated the early phase of the olfactory perception in discriminating among thousands of odorant molecules. However, very few ORs have been characterized from human origin. Consequently, the specificity and functional properties of individual OR still remains poorly understood, especially in humans.
In this study, human olfactory receptor (hOR) 1-60 was expressed on cell surface of human embryonic kidney (HEK)-293 cells as a fusion protein with rho-tag to its N-terminus for plasma membrane targeting.
The interactions between HEK-293 cells expressing hOR1-60 and various odorants elicited the change of Ca2+ ions and the change of Ca2+ ions was detected using ratiometric Ca2+ assay. Using Ca2+ assay, we found that hOR1-60 proteins are activated by esters. These findings will facilitate understanding about combinatorial code of hOR at the molecular level and our approach could be used as a tool for characterizing of other orphan hOR proteins.