earticle

영어

Hyaluronic acid (HA) and hyaluronidase have been implicated in cancers. The level of HA and hyaluronidase in cancer cells is higher than those in normal cells. The exact measurement of HA concentration and hyaluronidase activity, therefore, can be a potential diagnostic tool to detect cancers. The conventional methods to determine the amount of HA and hyaluronidase
activity are time-consuming and requiring expensive instruments. In this study, a novel method to measure HA concentration and hyaluronidase activity was developed on the basis of converging bio- and nano-materials.
Briefly, HA was conjugated to gold nanoparticles (AuNPs) to employ the color-changing property of AuNPs depending on interparticle distance. Thus, the AuNPs with amino surface (amino-AuNPs) easily formed AuNPs-HA complex through ionic interaction upon simple mixing the two component solutions. The formation of AuNPs-HA complex resulted in a color change of the solution depending on the HA concentration. The color change was easily detected by naked eyes or by UV/VIS spectrophotometer for more precise detection. In addition, the aggregation of amino-AuNPs caused by HA was also detected by transmission electron microscope (TEM). This phenomenon was also applied to determine the hyaluronidase activity. The details will be presented in the conference.