원문정보
초록
영어
Partitioning and precipitation processes have been used successfully for the separation, purification and analysis of various biological materials. The properties of the coexisting phases are important determinants of the partitioning of proteins in both precipitation and aqueous two-phase systems (ATPS).
In this investigation, the phase separation behavior of polyethyleneglycol (PEG) and PEGylated IgG with dextran based two-polymer systems have been studied.
The studies focus on the phase behavior in the presence of mouse anti-human IgG that affect the PEGylated IgG. The volume ratio of two phases in microchannel was compared based upon the infused volumetric flow rate of each polymer solution. Developed technique will be used for the concurrent phase-separation and affinity separation of biomolecules.