원문정보
초록
영어
We described a chip-based method for detecting protein glycosylation by using the conjugate of quantum dot (QD) and gold nanoparticle (AuNP). Our system relies on modulations in the energy transfer between two nanoparticles on a surface; that is, the photoluminescence (PL) of
lectin-coated QD (energy donor) immobilized on glass is quenched by carbohydrate-coated AuNPs (energy acceptor), and the presence of the glycoprotein causes the increase of the PL of QD. As a model system, we have shown that concanavalin A-conjugated QDs (ConA-QDs) and
dextran-modified AuNPs (Dex-AuNPs) can be used for the specific, quantitative detection of mannosylated BSA in terms of both the total concentration and the number of mannose residues on each BSA molecule.
When compared to conventional dye labels, the AuNPs not only showed a higher quenching efficiency as an energy acceptor, but also had a broader quenching ability towards QDs with different emission wavelengths. In addition, this chip-based format enabled no aggregation of nanoparticles and much smaller reaction volume. Our system will find applications for analysis
of glycoproteins with high selectivity and sensitivity in a high-throughput manner.