원문정보
초록
영어
Genitourinary infectious diseases are known to occur frequently and some of them have high
possibility to pass into chronic diseases. However, current treatments are carried out without
broad and exact screening of infected pathogens and antibiotic resistances, causing overdose
of multiple antibiotics. For the efficient diagnosis and treatment, we developed a DNA chip
for the multiplex detection of pathogens causing genitourinary infectious diseases.
In this study, we selected 14 pathogens which are known to cause major infections. Through
full consideration of their sequences, we settled the condition for multiplex PCR, and the
capture probe sequences. The amine modified capture probes were immobilized on the
aldehyde glass surface by our novel method, using gold nanoparticles. By using this method,
the immobilization efficiency increases, and at the same time, it is able to control the quality
of the manufactured DNA chip by silver enhancement. The target samples were labeled by
using Cy3 labeled primers. Then, the PCR products were applied to the manufactured DNA
chips, and the hybridization condition was optimized.
Through our research, we could get the correct signals from the single infection, and also
from the multiple infections simultaneously. Our DNA diagnostic chip is suitable for the
point-of-care testing due to its efficiency, convenience, and the eligible cost of money and
time.