원문정보
초록
영어
L-homophenylalanine is a building block of Angiotensin-Converting Enzyme(ACE) inhibitor and 2-phenylethanol is a most widely used aroma compound with rose-like odor. L-homophenylalanine and 2-phenylethanol were synthesized simultaneously from 2-oxo-4-butanoic acid and L-phenylalanine by coupling reaction of aminotransferase and the Ehrlich pathway using a recombinant E.coli. By adding on carbonyl reductase cofactor regeneration system, high yield L-homophenylalanine and 2-phenylethanol synthesis reaction is available. To select carbonyl reductase, the activities of six carbonyl reductases from Saccharomyces cerevisiae were assayed. Among those candidates, YGL157w showed the highest activity on phenylacetaldehyde. Phenylpyruvate decarboxylase was recruited from the Ehrlich pathway of Saccharomyces cerevisiae, which is the metabolic pathway of L-phenylalanine. In addition, regeneration of an expensive cofactor NADPH is achieved using Glucose dehydrogenase from Bacillus subtilis. Despite of the low kcat value of decarboxylase, 39.2mM of 2-phenylethanol was produced via whole cell reaction although we have used 1mM of NADPH.