원문정보
초록
영어
The reconstruction of rabbit corneal epithelium was performed through differentiation culture method using self-manufactured amniotic membrane (AM) supporter and lyophilized amniotic membrane (LAM). To evaluate the reconstructed corneal epithelium, we stained the reconstructed tissue sections against CK3, PCNA, and examined TEM. Tilting dynamic culture was better than static culture in terms of reconstructed corneal cell layers and proliferating
activity for reconstructing corneal epithelium on LAM. Blinking dynamic culture, comparing with static culture, showed similar properties with rabbit cornea in stratification and differentiation of corneal epithelial cells under histological examination. The corneal epithelium was successfully reconstructed by the use of LAM and self-manufactured AM supporter by blinking dynamic culture. The reconstructed corneal epithelium in this experimental study is considered to be a
good in vitro model in autograft and allograft for treating the patients with severely damaged corneal surface and allograft for treating damaged skin and mucosa.