원문정보
초록
영어
Our lab have tried to asymmetrically synthesize chiral amine, (s)-MBA with w-TA from Vibrio fluvialis JS17 using alanine and acetophenone as amine donor and amine acceptor respectively. But low equilibrium constant and product inhibition prevent from synthesizing highly concentrated (s)-MBA. The goals of this experiment is to analyze the problem of alanine, acetophenone reaction system and to find out the way to asymmetrically synthesize chiral amine compounds
with high-yield. We succeed to get purified PLP-Enzyme and PMP-Enzyme. The typical absorption peak of PLP-enzyme and PMP-enzyme is 420nm, 340nm in UV-visible spectra respectively Doing half reaction between purified PLP-enzyme and ala, we can observe how
absorption maxima is converted to counterpart. And, Doing half reaction between purified PMP-enzyme and acetophenone, the same as above. With these data we can find out the limit of alanine as amine donor and the limit of acetophenone as amine acceptor. Moreover, we can find out the way to synthesize divers chiral amine compounds with high-yield to circumvent low equilibrium problem. According the combination of relative activity of amine donor, amine acceptor, conjugated amine donor and conjugated amine acceptor, we classified 4 type reactions. To synthesize chiral amine compounds with high-yield, we conclude that we have to
consider the relative activity of amine donor, amine acceptor, conjugated amine donor and conjugated amine acceptor