원문정보
초록
영어
The genotype identification method to measure nucleic acid using is one of the interesting tools to detect E.coli in environmental samples. The four independent processes, that is, cell lysis, nucleic acid purification/concentration, nucleic acid amplification, and detection are required to detect E.coli. If micro chip format is utilized in genotype identification method, it also requires all processes required for the detection of E.coli because the volume reduction issue is key process to increase the detection sensitivity in environmental sample that has relative large
volume, considering microchip format. However, the washing issue might be emerged in nucleic acid isolation process, one of the four essential processes, when applying it to micro chip format because the most popular nucleic acid isolation process, called boom technology, utilizes chaotropic salt and ethanol as known as PCR inhibitors, which requires long washing step to remove chaotropic salt and high power usage such as centrifugal force to dry ethanol.
In this study, new method to isolate and concentrate nucleic acid is introduced for micro chip format to detect E.coli using microbead. Proposed method was optimized using RT-PCR and was well-demonstrated by measurement of ultra low concentration of E.coli without special reagent. Acknowledgments : This research was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-D00099) and by the Korea Science
and Engineering Foundation (KOSEF) grant funded by the Korea government(MOST) (No.2007-04373)