earticle

영어

Marine biofilm is composed of complex communities of microorganisms that interact with each other and their environment. The marine biofilms causeindustrial and economical problems. However, the developments of bacterial community in biofilm have not been studied in detail so far. The goals of this study were to assess the diversity and succession of bacterial communities involved in marine biofilm on three surfaces (acryl, glass, and steel substratum). The test surface differed in physicochemical properties was submerged in seawater for 36 hours with the sampling interval of 3 or 12 hours. We investigated the structure of bacterial communities of marine biofilms by terminal restriction fragment length polymorphism (T-RFLP) and the nucleotide sequences of 16S rRNA genes. The succession from the initial to late communities was observed at 12 hours on three surfaces by T-RFLP analysis. The diversity index appeared that the value of late stage was higher than that of initial stage in the acryl surface. The analysis of the nucleotide sequences of 16S rRNA genes identified from the biofilm showed that γ-proteobacteria were predominant in the community of initial stages, and the ratio of α-proteobacteria were increased as the biofilm develops into late stages. The major clones of 16S rRNA library in the biofilm on the acryl surface were affiliated with Pseudomonas sp. and Acinetobacter sp. of γ-proteobacteria and appeared to be involved in the initial stage within the 12 hours, although the distinct structure of biofilm on the steel surface was not observed. In conclusion, γ-proteobacteria and α-proteobacteria play an important role in development and succession of biofilm on artificial surfaces. [Supported by grants from MarineBio21]