원문정보
초록
영어
PNA (Peptide Nucleic Acid) is synthetic DNA analogues with neutral polypeptide backbone. The PNA probes enhance thermal stability, resistance to the nuclease and protease, stronger and faster binding affinity to the complementary DNA or RNA.
These remarkable hybridization properties of PNA suggest that PNA probes may be efficiently incorporated into microarrays.
We have developed a PNA array platform technology for HPV genotyping, detection of lamivudine-resistant HBV, CYP 450 SNP genotyping, and miRNAs expression profiling.
PNA arrays have a greatly increased specificity and sensitivity, and extended shelf-life relative to DNA arrays.
We have also developed FLASH (Fluorescent Labeling After Speedy Hybridization) technology on PNA array. The FLASH allows that only hybridized target DNAs can be labeled with fluorescence dye after PNA-DNA hybridization. Specificity of the PNA array was achieved by combining FLASH technology into the assay.
PNA array platform technology provides a powerful and reliable assay for SNP genotyping and gene expression profiling. Therefore, PNA array is expected to play a vital role in diagnostic applications.