earticle

영어

The covalent coupling of antibodies to solid surfaces or other biological/chemical molecules is a key step for the development of immune-based assays such as biosensors and antibody arrays. The choice of coupling method significantly affects subsequent antibody-antigen interactions. In order to fully maintain antigen binding abilities of modified antibodies, modifications must be site-selective and away from antigen binding sites, providing well-oriented antibodies.
Antibody binding protein, protein G specifically binds the Fc region of an antibody and therefore provides proper orientation of the bound antibody, resulting in the antigen binding sites optimally-exposed to the assay solution without the need of any antibody modifications. Here we introduce several novel linker systems which utilize engineered protein G constructs for antibody immobilization. Various genetic and/or chemical modifications of protein G allowed controlled and efficient immobilization of antibodies on the surfaces of different assay platforms, such as those of glass/gold chips and nanoparticles.