earticle

영어

There have been many attempts to develop sensitive and accurate techniques for the detection and diagnosis of pathogenic bacteria using nucleic acid-based technology. To achieve efficient simultaneous detection of eleven selected pathogens, we constructed oligonucleotide microarray containing double specific capture probes and developed a statistical pattern mapping analytical tool for pathogenic bacteria detection. The two capture probes were designed from the total variable regions of 16S rDNA for precise detection. This microarray system harboring double capture probes and pattern recognition showed precise subtype discrimination between two closely related species. Therefore, using the proposed oligonucleotide microarray, we could successfully classify species and even subtypes of food-borne pathogens simultaneously.