원문정보
초록
영어
Enzymes are necessary for maintaining all living systems and also can catalyze a number of chemical reactions of possible biotechnological importance. Some major research and technology areas are needed to develop the biotechnology-based production of valuable products. These areas include, for example, techniques for the screening, expression, evolution, production, immobilization and application of enzymes. Among a variety of enzymes as
biocatalysts, lipases, which hydrolyze and synthesize the ester bond of a large number of natural and synthetic ester substrates, are ubiquitous enzymes found in animals, plants, and microorganisms. They can catalyze the hydrolysis of triglycerides to glycerol and fatty acids at the lipid-water interface and the synthetic applications in non-aqueous medium such as
transesterification of triglycerides and resolution of racemic mixture. We will provide discussions concerning above-mentioned major research and technology areas to develop the in-house lipase biocatalysts for successful industrial applications; isolation of novel lipases, directed evolution of lipase-secreting apparatus, mass-production of extracellular lipase,
immobilization of lipase to synthetic polymeric carriers and lipase-catalyzed reactions.