원문정보
초록
영어
Temperature is one of the key environmental parameters that affect the growth of Chinese hamster ovary (CHO) cells and the production of recombinant proteins. Generally, CHO cells are cultivated at 37°C to simulate the body environment where they are familiar. Recently, a number of studies have demonstrated that lowering culture temperature below 37°C is beneficial to specific productivity (q) in CHO cells although it decreases specific growth rate (µ). To investigate the effect of low culture temperature on cell growth and recombinant protein productions, CHO cells producing erythropoietin (EPO) and follicle-stimulating hormone (FSH) were cultivated between 28ºC to 37ºC with various culture modes such as batch, fed-batch and perfusion. Lowering the culture temperature below 37ºC suppressed cell growth but enhanced qEPO and qFSH. Also, the quality of EPO produced under different culture modes was investigated through isoform analysis. Among the culture modes tested, perfusion seems to be promising in terms of both quantity and quality of proteins.