earticle

영어

Yeast is a good host for the expression of complicated mammalian proteins because yeast, like mammalian cells, has quality control system in the secretion pathway which can perform eukaryotic-specific proteolytic processing, chaperone-assisted folding and posttranslational modifications, such as disulfide-bond formation and certain types of glycosylation. Thus, yeast surface display system combined with high throughput screening techniques has been extensively employed as a platform technology for the protein and antibody engineering for affinity maturation with their ligands/antigens, higher stability and expression efficiency in yeast. However, yeast surface display system has been little explored to express cDNA library from some tissues/cells to isolate novel antigens or biomarkers. In this talk, I will present two topics related with antibody engineering and biomarker discovery using yeast surface display system. First, taking a specific example, I will introduce the construction of so called pseudo-immune antibody library to increase the probability of isolating a good binder antibody to a given antigen. Secondly, I will present a selection methodology to isolate patient sera-specific antigens from yeast surface-expressed cDNA library constructed from 15 patient lung tissues with non-small cell lung cancer. [This work was partially supported by grants from the National R&D Program for Cancer Control, Ministry of Health & Welfare (0520110-1 to YSK), Korea Research Foundation (R08-2004-000-10077-0 to YSK), and the "GRRC" Project of Gyeonggi Provincial Government (to YSK), Republic of Korea.]