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Research Notes

Heterologous Gene Expression of aprE2 Encoding a 29kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716

원문정보

Gun-Hee Kwon, Woo-Ju Jeong, Ae Ran Lee, Jeong Hwan Kim, Jae-Yong Park, Jaeho Cha, Young-Sun Song

한국식품과학회 Food Science and Biotechnology Volume 17 Number 6 2008.12 pp.1372-1375
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초록

영어

The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using aBacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantlycompared to B. licheniformis 10716 control cell. During the 100hr incubation in Luria-Bertaini broth at 37oC, fibrinolyticactivity of B. licheniformis TF increased rapidly at the late growth stage, after 52hr of incubation, which was confirmed byzymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media,60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

저자정보

  • Gun-Hee Kwon Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea
  • Woo-Ju Jeong Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea
  • Ae Ran Lee Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea
  • Jae-Yong Park Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea
  • Jaeho Cha Department of Microbiology, Pusan National University, Busan 609-735, Korea
  • Young-Sun Song School of Food and Life Science, Inje University, Gimhae, Gyeongnam 621-749, Korea
  • Jeong Hwan Kim Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea

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