원문정보
초록
영어
The purpose of this study was to characterize and investigate the immune activity of CpG oligodeoxynucleotides(ODNs) from Bifidobacterium longum. Bacterial CpG motifs have attracted considerable interests because of theirimmunomodulatory activities. Genomic DNA from B. longum was prepared and amplified for 4 different 180-188-merdouble-stranded ODNs (BLODN1-BLODN4). When immune cells (RAW 264.7 murine macrophages and JAWS II dendriticcells) with these ODNs were treated, BLODN4 induced the highest immune activity. To assess the effectiveness of the CpGsequences within BLODN4, single-stranded 40-mer ODNs containing CpG sequences (sBLODN4-1, sBLODN4-2) weresynthesized. sBLODN4-1 induced higher level of cytokines such as interleukin (IL)-12p40 and tumor necrosis factor (TNF)-αby macrophage and IL-6 and TNF-α by dendritic cells than did sBLODN4-2. The results suggest that CpG ODNs-enrichedcomponents of B. longum might be useful as an immunomodulatory functional food ingredient.
목차
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
저자정보
참고문헌
- 1Alvarez ME, (2006) Neutrophil signaling pathways activated by bacterial DNA stimulation, Arch. Immunol. Ther. Ex
- 2Macrophages ingest and are activated by bacterial DNA.네이버 원문 이동
- 3Probiotics: from myth to reality. Demonstration of functionality in animal models of disease and in human clinical trials네이버 원문 이동
- 4Klijn A, (2005) Lessons from the genomes of bifidobacteria, FEMS Microbiol. Rev
- 5Bacterial DNA and immunostimulatory CpG oligonucleotides trigger maturation and activation of murine dendritic cells.네이버 원문 이동
- 6Bifidobacterium breve and Streptococcus thermophilus secretion products enhance T helper 1 immune response and intestinal barrier in mice.네이버 원문 이동
- 7Supernatant of Bifidobacterium breve induces dendritic cell maturation, activation, and survival through a Toll-like receptor 2 pathway네이버 원문 이동
- 8Lammers KM, (2003) Immunomodulatory effects of probiotic bacteria DNA: IL-1 and IL-10 response in human peripheral blood mononuclear cells, FEMS Immunol. Med. Mic
- 9DNA from probiotic bacteria modulates murine and human epithelial and immune function네이버 원문 이동
- 10Amplification of antibody production by phosphorothioate oligodeoxynucleotides네이버 원문 이동
- 11Activation of human B cells by phosphorothioate oligodeoxynucleotides.네이버 원문 이동
- 12The Genome Sequence of Bifidobacterium longum Reflects Its Adaptation to the Human Gastrointestinal Tract네이버 원문 이동
- 13Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption.네이버 원문 이동
- 14Lactobacillus rhamnosus induces peripheral hyporesponsiveness in stimulated CD4+ T cells via modulation of dendritic cell function.네이버 원문 이동
- 15Probiotics ameliorate recurrent Th1-mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-beta-bearing regulatory cells.네이버 원문 이동
- 16Oral probiotic bacterial administration suppressed allergic responses in an ovalbumin-induced allergy mouse model네이버 원문 이동
- 17Allergy in marathon runners and effect of Lactobacillus GG supplementation on allergic inflammatory markers네이버 원문 이동
- 18Dietary Probiotic Supplementation Enhances Natural Killer Cell Activity in the Elderly: An Investigation of Age-Related Immunological Changes네이버 원문 이동