earticle

영어

The purpose of this study was to characterize and investigate the immune activity of CpG oligodeoxynucleotides(ODNs) from Bifidobacterium longum. Bacterial CpG motifs have attracted considerable interests because of theirimmunomodulatory activities. Genomic DNA from B. longum was prepared and amplified for 4 different 180-188-merdouble-stranded ODNs (BLODN1-BLODN4). When immune cells (RAW 264.7 murine macrophages and JAWS II dendriticcells) with these ODNs were treated, BLODN4 induced the highest immune activity. To assess the effectiveness of the CpGsequences within BLODN4, single-stranded 40-mer ODNs containing CpG sequences (sBLODN4-1, sBLODN4-2) weresynthesized. sBLODN4-1 induced higher level of cytokines such as interleukin (IL)-12p40 and tumor necrosis factor (TNF)-αby macrophage and IL-6 and TNF-α by dendritic cells than did sBLODN4-2. The results suggest that CpG ODNs-enrichedcomponents of B. longum might be useful as an immunomodulatory functional food ingredient.