earticle

영어

The purpose of this study was to investigate the effects of Kyung Hee Allergic Disease Herbal Formula (KAHF)on atopic dermatitis (AD) and its mode of action. Our clinical study showed KAHF reduced Severity Scoring of AtopicDermatitis (SCORAD) indexes and subjective symptom scores. In parallel, the decreased levels of interferon (IFN)-γ andinterleukin (IL)-5 in serum, which contributed to its AD-mitigating effect was observed. To reveal the underlying mechanismsof KAHF in AD, its anti-inflammatory effect on lipopolysaccharide (LPS)-induced responses in RAW 264.7 cells wasexamined. KAHF was found to significantly inhibit the productions of nitric oxide (NO), prostaglandin E2 (PGE2), and IL-1βin LPS-stimulated RAW 264.7 macrophages. Consistently, KAHF potently inhibited protein and mRNA expressions ofinducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, KAHF inhibited LPS-induced activationof nuclear factor (NF)-κB. Taken together, our data suggest that KAHF has a beneficial effect on several eicosanoid-relatedskin inflammations, such as atopic dermatitis.