earticle

영어

The aim of the present study was to prepare nanosample of fucoidan using lecithin as encapsulated material and toinvestigate the anticancer and immunomodulatory activity of nanoparticle in vitro. The nanoparticles have been characterizedby dynamic light scattering (DLS) and transmission electron microscopy (TEM). Confocal microscopy confirmed theinternalization of the fucoidan conjugates into the immune cells. The uptake of nanoparticles was confirmed with confocalmicroscopy demonstrating their localization in the cells. The anticancer activity was increased over 5-10% in different cancercells of fucoidan nanoparticle as compare with fucoidan. The human B and T cells growth and the secretion of interleukin-6and tumor necrosis factor-α from B cell were also improved by fucoidan nanoparticle because of the rapid absorption ofnanoparticle into the cells as compare to fucoidan. At 0.6mg/mL concentrations, the fucoidan nanoparticle showed betteractivity than 1.0mg/mL concentration in T cell growth because the cells reached their saturation capacity. When the fucoidanwas encapsulated in lecithin, its anticancer as well as its immunomodulatory activity proved to be superior from that of itselfin pure form.