earticle

영어

Having idea to develop more effective anti-diabetic agent from ginseng root, we comprehensively assessed theanti-diabetic activity and mechanisms of ginsam in C57BL/KsJ db/db mice. The db/db mice were divided into 4 groups;diabetic control (DC), ginsam at a dose of 300 or 500mg/kg (GS300 or GS500) and metformin at a dose of 300mg/kg(MT300). Ginsam was orally administered for 8 weeks. GS500 reduced the blood glucose concentration and significantlydecreased an insulin resistance index. In addition, GS500 reduced the plasma non-esterified fatty acid, triglyceride, andincreased high density lipoprotein-cholesterol as well as decreased the hepatic cholesterol and triglyceride. More interestingly,ginsam increased the plasma adiponectin level by 17% compared to diabetic control group. Microarray, quantitative-polymerase chain reaction and enzyme activity results showed that gene and protein expressions associated with glycolysis,gluconeogenesis, and fatty acid oxidation were changed to the way of reducing hepatic glucose production, insulin resistanceand enhancing fatty acid β-oxidation. Ginsam also increased the phosphorylation of AMP-activated protein kinase and glucosetransporter expressions in the liver and skeletal muscle, respectively. These changes in gene expression were considered to bethe mechanism by which the ginsam exerted the anti-diabetic and anti-dyslipidemic activities in C57BL/KsJ db/db mice.