earticle

영어

Secondary metabolites from the fruit body of Phellinus linteus were evaluated for their proliferative effect onhuman osteoblast-like cells. 3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenyl-tetraxolium bromide (MTT) assay and alkaline phosphatase(ALP) activity assay were used to assess the effect those isolates on the human osteoblast-like cell line (Saos-2). Activity-guided fractionation led to the isolation of ALP-activating phenolic compounds through the extraction of P. linteus, solventpartitioning, and repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations. From the result ofspectroscopic data including nuclear magnetic resonance (NMR), mass spectrometry (MS), and infrared spectroscopy (IR), thechemical structures of the compounds were determined as 4-(4-hydroxyphenyl)-3-buten-2-one (1), 2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), 3,4-dihydroxybenzaldehyde (4), and protocatechuicacid methyl ester (5), respectively. This study reports the first isolation of compounds 1-3 and 5 from P. linteus. In addition, allphenolic compounds stimulated proliferation of the osteoblast-like cells and increased their ALP activity in a dose-dependentmanner (10-8 to 10-1 mg/mL). The present data demonstrate that phenolic compounds in P. linteus stimulated mineralization inbone formation caused by osteoporosis. The bone-formation effect of P. linteus seems to be mediated, at least partly, by thestimulating effect of the phenolic compounds on the growth of osteoblasts.