earticle

영어

This study was undertaken to classify Enterobacter sakazakii isolates from 13 powdered infant formula products,25 powdered weaning diet products, and 33 weaning diet ingredients on polymerse chain reaction (PCR) methods. Thenumbers of the isolates from 1 powdered infant formula product, 7 powdered weaning diet products, and 6 weaning dietingredients were 1, 14, and 8, respectively. The contaminated ingredients were 1 rice powder, 2 millet powders, 2 vegetablepowders, and 1 fruit and vegetable premix. PCR with the primer of repetitive extragenic palindromic element (REP-PCR) andrandom amplification of polymorphic DNA(RAPD) were effective in discriminating among the isolates, but tRNA-PCR andPCR with the primer of 16S-23S internal transcribed spacer (ITS-PCR) were not. Some of E. sakazakii isolates from vegetablepowders, fruit and vegetable premix, and millets powders were classified into the clonal groups based on the DNA patterns inthe REP-PCR and RAPD analysis. A close genetic relationship among the isolates from some of the powdered weaning dietproducts and the rice powder was also detected in the cluster analysis based on the DNA patterns in RAPD.