원문정보
초록
영어
In this study, we examined the effect of hydroxyl radical generated by -ray and UV irradiation on shrinkage of vitreousbody. Change in gel ratio of vitreous body and change in the properties of its components (collagen, sodium hyaluronate)were analyzed. By comparing these results, the amount of hydroxyl radical, which induces the considerable shrinkage ofvitreous body, was evaluated from theoretical calculation based on experimental condition and some reported kineticparameters. It was concluded that the integrated amount of hydroxyl radical required to liquefy half of the vitreous body(Vitreous body gel ratio = 50%) was estimated as 140 µmolg-1from -ray irradiation experiment. Also, from UVirradiation experiment result, it was confirmed that the effect of hydroxyl radical is larger than that of other reactive species.The causes of shrinkage of vitreous body are supposed as follows, 1) decrease in viscosity by cleavage of glycoside bondin sodium hyaluronate, 2) leaching of collagen from vitreous body and 3) leaching of crosslinked products and scissionproducts of collagen.
목차
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1 Sample Preparation
2.2 Irradiation
3. ANALYSIS PROCEDURE
3.1 Vitreous Body Gel Ratio and Change in Molecular Weight of Collagen in Vitreous Body
3.2 Change in Thickness of Collagen Gel
3.3 Change in Viscosity of Sodium Hyaluronate Solution
4. RESULTS AND DISCUSSION
4.1 Change in Gel Ratio of Vitreous Body by r-ray Irradiation
4.2 Result of model experiment
4.3 Measurement of Molecular Weight of Ollagen in Vitreous Body and Vitreous Humor by Electrophoresis after r-ray Irradiation
5. CONCLUSION
REFERENCES