원문정보
초록
영어
The present study investigated the effects of follicle stimulating hormone (FSH) and human chorionic gonadotrophin (hCG) on the nuclear maturation of canine oocytes. Oocytes were recovered from mongrel female ovaries in various reproductive states; follicular, luteal or anestrous stage. Oocytes were cultured in serum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.5, 1.0 or 10 IU) or hCG (Exp. 2: 0, 0.5, 1.0 or 10 IU) or both (Exp. 3: 1 IU FSH + 1 IU hCG) for 72 hr to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting. The denuded oocytes were stained with 1.9 μM. Hoechst 33342 in glycerol and the nuclear state of oocytes was evaluated under UV light. More (p<0.05) oocytes matured to MII stage when follicular stage oocytes were supplemented with 1 IU FSH (6.2%) compared with the control, 0.1 or 10.0 IU FSH (0 to 1.2%). Significantly higher (p<0.05) maturation rate to MII stage was observed in follicular stage oocytes supplemented with 1.0 IU hCG (7.2%) compared with the control or other hCG supplemented groups (0 to 1.5%). However, the combination of FSH and hCG did not improve the nuclear maturation rate of canine oocyte (2.4 %) compared with FSH (6.2%) and hCG alone (7.2%). In conclusion, FSH or hCG alone significantly increased the maturation of canine oocytes to MII stage.
목차
INTRODUCTION
MATHRIALS AND METHODS
Collection of Canine Oocytes
In Vitro Maturation
Assessment of Meiotic Stage
Statisical Analysis
RESULTS
Exp. 1: Effect of FSH Supplementation on Nuciear Maturation
Exp. 2: Effect of hCG Supplementetion on Nuclear Maturation
Exp. 3: Effect of Combined Supplemantation of FSH and hCG on Nuclear Maturation
DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
저자정보
참고문헌
- 1(1997) Mechanical isolation and in vitro growth of preantral and small antral human follicles,
- 2Cytoplasmic microtubular dynamics and chromatin organization during mammalian oogenesis and oocyte maturation.네이버 원문 이동
- 3The Appearance and Structure of Intercellular Connections during the Ontogeny of the Rabbit Ovarian Follicle with Particular Reference to Gap Junctions네이버 원문 이동
- 4Gap Junctions between the Oocyte and Companion Follicle Cells in the Mammalian Ovary네이버 원문 이동
- 5(1997) Follicle stimulating hormone inhibits apoptosis in pre- and early-antral murine follicles in vitro,
- 6(1991) : Cellular localization and hormonal regulation of follicle-stimulating hormone and luteinizing hormone receptor messenger RNAs in the rat ovary. Mol. Endocrinol 5:1405-1417.,
- 7Gonadotropin suppression of apoptosis in cultured preovulatory follicles: mediatory role of endogenous insulin-like growth factor I.네이버 원문 이동
- 8(1996) Hormonal regulation of apoptosis in early antral follicles: follicle stimulating hormone as a major survival factor,
- 9Biology and endocrinology of ovulation, pregnancy and parturition in the dog.네이버 원문 이동
- 10Biology of gonadotrophin secretion in adult and prepubertal female dogs.네이버 원문 이동