원문정보
초록
영어
β-Glucuronidases of intestinal bacteria are capable of retoxifying compounds that have been detoxified by liver glucuronidation, which is one of the most important detoxication processes in the liver. Therefore, this enzyme is known to accelerate colon cancer invasion and metastasis. Two bromophenols, 2,4,6-tribromophenol (I) and 2,4-dibromophenol (II), were purified from the red alga Grateloupia elliptica. IC50 values of bromophenol I and II against Escherichia coli β-
glucuronidase were 5.4 and 8.5 mg/mL, respectively. Hence, bromophenols of G. elliptica, a potent β-glucuronidase inhibitor, can be used as a novel pharmaceutical agent for the prevention and treatment of colon cancer.
목차
Introduction
Materials and Methods
Reagents
Algal samples
Extraction and purification of β-glucuronidase inhibitors
Inhibitory assay of β-glucuronidase activity
Identification of β-glucuronidase inhibitor
2,4,6-Tribromophenol (I)
2,4-Dibromophenol (II)
Statistical analysis
Results and Discussion
Extraction and isolation of β-glucuronidase inhibitor
Purification and identification of β-glucuronidaseinhibitor
Inhibitory activity of bromophenols against β-glucuronidase
References