원문정보
초록
영어
To investigate the antiviral activity of marine algae against fish pathogenic viruses, which are often the causes of viral disease in aquaculture, the 80% methanolic extracts of 21 species collected from the coast of Korea were screened for their in vitro antiviral activities on infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), using a flounder spleen (FSP) cell-line. Among them, Monostroma nitidum (10 μg/mL) exhibited the strongest inactivation on IHNV, showing a 2 log reduced virus titre as compared to the control in the determination of direct virucidal activity. In addition, Polysiphonia morrowii (100 μg/mL) remarkably reduced the virus titres of treated cells by 2-2.5 log, for both IHNV and IPNV, in the determination of cellular protective activity, implying the existence of substances that may
modulate innate host defense mechanisms against viral infections. These results reveal that some marine algae could be promising candidates as sources of antiviral agents or as health-promoting feeds for aquaculture.
목차
Introduction
Materials and Methods
Algae
Preparation of algae extracts
Viruses and cell culture
Cytotoxicity assay
Assessment of antiviral activity
Results and Discussion
Antiviral activities of 21 species of Korean marine algaeextract against IHNV and IPNV
Constituents responsible for the antiviral activitiesagainst IHNV and IPNV from algae
References