원문정보
초록
영어
Overexpression of HER2 in breast cancer cells is considered to induce the expression of acetyl-CoA carboxylase α (ACACA) and fatty acid synthase (FASN) through activation of mammalian target of rapamycin (mTOR) signaling pathway.
Resveratrol, a red wine polyphenol, has been shown to induce apoptosis in several cancers by interfering in several signaling pathways. Present study elucidated the mechanism by which resveratrol downregulates ACACA and FASN in breast cancer cells. Resveratrol activated AMP-activated protein kinase (AMPK) and downregulated mTOR in BT-474 cells. These effects
of resveratrol were mimicked by AICAR, an AMPK activator, and exogenously expressed constitutively active AMPK, while they were abolished by a dominant-negative mutant of AMPK. The downregulation of mTOR was not accompanied with changes in Akt, the upstream regulator of mTOR. These findings indicate that the downregulation of ACACA and FASN by resveratrol is mediated by the downregulation of mTOR signaling pathway via activation of AMPK.
목차
Introduction
Materials and Methods
Reagent
Cell culture
Cell viability assay
Immunoblot analyses
Reverse transcription (RT)-polymerase chain reaction(PCR)
Results and Discussion
Resveratrol decreases viability of breast cancer cellsoverexpressing HER2
Effects of resveratrol on the expression of lipogenicenzymes and on activation of signaling molecules
Resveratrol-mediated activation of AMPK downregulatesmTOR signaling pathway
References