원문정보
초록
영어
This study was performed to determine the effects of γ-irradiation on the antiallergic activity of alginate. An alginate aqueous solution was γ-irradiated at 3, 5, 7, 10, 20, and 100 kGy. First, the molecular weight (Mw) of alginate rapidly decreased as the γ-irradiation dose increased up to 20 kGy. Then, the antiallergic activity of the γ-irradiated alginate was measured. Interlukin (IL)-4 cytokine and IgE level were significantly decreased in the γ-irradiated alginate groups as
compared to the control group in vitro. The mice intraperitoneally administered with the γ-irradiated alginate exhibited lower ovalbumin-specific IgE and IgG1 level in serum than the control mice. Furthermore, the γ-irradiated alginate suppressed total and ovalbumin-specific IgE secretions in the splenocytes. Increased IL-2 level was observed in the culture supernatants of the splenocytes that were obtained from the mice administerd with the γ-irradiated alginate, while IL-4 level decreased. The present study indicates that γ-irradiated alginate can suppress allergy in a mouse allergy model.
목차
Introduction
Materials and Methods
Mice
Reagent
γ-Irradiation
Gel permeation chromatography (GPC)
Immunization protocol
Spleen cell culture for IgE and cytokine production
Determination of cytokine level
Determination of ovalbumin-specific antibody level
Splenocytes proliferation assay
Statistical analysis
Results and Discussion
Effect of the γ-irradiation on the Mw of alginate
Effect of the γ-irradiated alginate on IL-4 and IgE levelin vitro
Effect of the γ-irradiated alginate on antibody level inthe serum
Effect of the γ-irradiated alginate on cytokine level inthe splenocytes
Effect of the γ-irradiated alginate on total andovalbumin-specific IgE in the splenocytes
Effect of the γ-irradiated alginate on the splenocytesproliferation
References